A Weibull Model To Describe Antimicrobial Kinetics Of Oregano And Lemongrass Essential Oils Against Salmonella Enteritidis In Ground Beef During Refrigerated Storage

The antimicrobial effect of oregano (. Origanum vulgare L.) and lemongrass (. Cymbopogon citratus (DC.) Stapf.) essential oils (EOs) against Salmonella enterica serotype Enteritidis in in vitro experiments, and inoculated in ground bovine meat during refrigerated storage (4. ±. 2°C) for 6. days was evaluated. The Weibull model was tested to fit survival/inactivation bacterial curves (estimating of p and δ parameters). The minimum inhibitory concentration (. MIC) value for both EOs on S. Enteritidis was 3.90μl/ml. The EO concentrations applied in the ground beef were 3.90, 7.80 and 15.60μl/g, based on MIC levels and possible activity reduction by food constituents. Both evaluated EOs in all tested levels, showed antimicrobial effects, with microbial populations reducing (. p≤. 0.05) along time storage. Evaluating fit-quality parameters (. RSS and RSE) Weibull models are able to describe the inactivation curves of EOs against S. Enteritidis. 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Comparação entre dois protocolos para obtenção de plasma rico em plaquetas, em cães

Avaliaram-se dois protocolos para a produção de plasma rico em plaquetas (PRP) com o sangue de 20 cães adultos. Foram coletados três frascos de sangue em que um deles foi usado para produção do PRP por meio do protocolo A - centrifugação única a 1200rpm/10min -, o outro para fabricação do PRP pelo protocolo B - primeira centrifugação a 1200rpm/10min e a segunda centrifugação a 1600rpm/10min - e o terceiro para realização da contagem plaquetária no sangue total, que serviu de parâmetro para os valores alcançados no PRP. O protocolo no qual foi possível alcançar maior concentração plaquetária foi testado em outros 20 cães para avaliar sua reprodutibilidade. Constatou-se que o protocolo B resultou em maior plaquetometria em 100% das amostras e concluiu-se ser ele eficiente para a produção do PRP em cães